Specialization

Cancer Biomarkers & Molecular Epidemiology, Respiratory Genetics

Email

siddharthsharma.phd@thapar.edu; Date of Joining: 01-07-2011

 Siddharth Sharma, PhD

 Professor

Department of Biotechnology                                              E-mail: siddharthsharma.phd@thapar.edu

Thapar Institute of Engineering and Technology, Patiala       Contact no: 9501688366

 

Research Interest

• SNP related Risk assessment, Cancer Biomarker discovery, Polymorphism relation to cancer susceptibility and clinical outcomes.
• Molecular markers for lung cancer prognosis, Protein Microarrays
• Genetics of pulmonary diseases like COPD and asthma
• Inflammation and chronic disease

Educational Qualification

• Ph.D (Cancer Genetics):  Panjab university, Chandigarh (2004)
• M.Sc (Life Sciences): School of Life Sciences, Pondicherry University (1998)
• B.Sc (Botany): Madras University (1996)

 

Position and Employment (Starting with the most recent employment)

S. No.	Institution Place	Position	From (Date)	To (date)
1	Department of Biotechnology, Thapar Institute of Engineering & Technology	Associate Professor	July 2017	Till date
2	Department of Biotech and Environment Sciences, Thapar University	Assistant Professor	July 2011	July 2017
3.	Beckman Coulter Pvt. Ltd	Application Scientist	August 2009	June 2011
4.	Department of Pathology, Miller School of Medicine, University of Miami Florida, USA	Postdoctoral Research Fellow	January 2007	June 2009
5.	Department of Molecular and cellular biology University of Cape Town	Postdoctoral Research Fellow	January 2005	Nov 2006
6.	Ranbaxy Research Laboratory, Gurgaon	Research Scientist	June 2004	Nov 2004
7.	NIPER, Mohali	Research Associate (Scientist Grade II)	Sept 2002	June 2004

 

 

 

 

 

 

 

 

 

 

Research Summary

Dr Siddharth Sharma, Associate Professor in Biotechnology, T.I.E.T has completed his undergraduate and post-graduate studies in Life Sciences from prestigious Institutes like University of Madras and Central University, Pondicherry. He then completed his doctoral studies in the area of Cancer genetics from Panjab University. His doctoral work won him the young scientist award in 2002 from Panjab Academy of Sciences.  After completion of his Ph.D he joined National Institute of Pharmaceutical and Educational Research (NIPER), Mohali as a Research Associate (Scientist Grade II) where he worked in the area of aging. After his stint in NIPER he joined Ranbaxy Laboratories as a research Scientist where he was involved in cloning and expression of Muscarinic receptors. To further hone his skills in research he joined as a post-doctoral fellow in the Institute of Infectious diseases and Molecular Medicine University of Cape Town and worked in the area of protein Microarrays of drug metabolizing genes, his Post-doctoral Fellow ship was sponsored from Procognia Limited a London based biotechnology company. After his completion of post-doctoral studies in Cape Town, he moved to USA and was appointed as post-doctoral fellow in the department of Pathology, Miller School of Medicine, University of Miami, USA. He worked in the area of hematopoietic stem cells. Dr Sharma, after completion of his PDF in USA joined as an application scientist in Beckman Coulter where he was involved in training on Flow Cytometry to scientists. In 2011 Dr Sharma joined Thapar Institute of Engineering and Technology as an assistant professor.

 

 

Current Research

Our research is focused on Genomic profiling of lung cancer – a) Risk assessment (b) Biomarker expression related especially clinical outcomes (c) Toxicogenomics in lung cancer (d) Genetic variants in signalling pathways and its role in pathogenesis of lung cancer (e) Genetics of COPD and Asthma

(a) Our primary area of research is related to the molecular epidemiology of lung tumors in North Indian population. Since genetic background has an important role in susceptibility to complex diseases, our work has helped us to identify key Single Nucleotide Polymorphism (SNPs) which are associated with individual susceptibility for lung carcinogenesis. Secondly, we have also identified the role of polymorphic variants of certain genes and their synergistic interaction with environmental stimuli. Thus, we are better able to understand the mechanisms through which environmental exposure interacts with cancer susceptibility genes which might open up new avenues for disease prevention. Current efforts are focused on polymorphisms in genes involved in DNA synthesis and repair. We have used a comprehensive genomic approach to assess the role of DNA repair capacity (DRC) in lung cancer etiopathogenesis and performed lung cancer risk assessment in hospital-based case–control studies. Our work has led us to the identification of interaction between tobacco smoke exposure and genetically susceptible subgroups that cause disease. This could further lead to the development of public health programs for protecting susceptible populations, and for targeted screening of groups at higher risk of disease.

(b) Another area of research where we are focusing is the role of SNPs and clinical outcomes in lung cancer patients. Lung cancer is the most common cancer amongst male malignancy and the overall survival rates are quite poor. Our primary area of research has been single nucleotide polymorphisms and tumor biomarkers and their correlation with treatment (Chemotherapy) outcomes and prognosis. Single nucleotide polymorphisms (SNPs) are one of the potential molecular markers that shall help us identify patients with unfavorable prognostic factors as well as those with poor response to chemotherapy, and therefore it might help in the selection of an appropriate chemotherapy regimen in the adjuvant and metastatic setting. We have identified SNPs of genes involved in the multistep processes of cancer genesis and the metabolism of chemotherapy that might prove clinically significant

 

(c) Recently our group has also delved in the area of the role of polymorphisms in relation to pharmacogenetics applications and personalized cancer therapy.  Since most of the chemotherapeutic agents for cancer treatment affect a minority of cancer patients and have a narrow therapeutic index that frequently causes life-threatening toxicities and even death. Thus, novel treatment strategies that can increase the effectiveness of therapy and decrease the rate of adverse events will be developed. We have identified SNPs of genes involved in playing an important role in profiling the toxicity of lung cancer patients undergoing chemotherapy. Under this approach, the aim of personalized medicine is to tailor the therapy options according to patient’s molecular profile.

(d) We have studied the role of genetic variations in the Wnt and AhR signalling pathway and evaluated its effect on lung cancer risk in North Indians. Our study is a primary effort to introduce the concept of polygenic approach to get an insight about the various polymorphic variants in determining cancer susceptibility. Our findings might help in the establishing the influence of genetic variants in the Wnt signaling pathway genes and its role towards lung carcinogenesis.

 Dr siddharth has active research collaborations with Department of Pulmonary Medicine PGIMER, Chandigarh and also with Rajindra Medical college and Hospital Patiala. He has published 45 research papers in international Journals of repute.

 

RESEARCH ACCOMPLISHMENTS

1. SPONSORED RESEARCH PROJECTS COMPLETED: 48 lacs INR from Govt. agencies.
2. DEVELOPED –Collaborative research projects with Department of pulmonary medicine, PGIMER, Chandigarh and Rajindra Medical college, Patiala
3. Leading an independent research group in the area of Lung cancer Biomarker discovery, Etiopathogenesis of lung and breast cancer
4. Scientific contribution includes >47 peer reviewed international publications (SCI); 4 Book chapters
5. Research networking: Department of pulmonary medicine, PGIMER, Chandigarh and Department of Pathology, Rajindra Medical college, Patiala

 

                             Ph.D. and Graduate students (from all sources) working at present with the mentor

Thesis supervision	Completed	Ongoing	Thesis submitted
PhD	7	6
DM	1
MD	2	1
M.Tech	14	0
M.Sc	29	0

 

 

• Publications of Dr. Siddharth Sharma (PI) (SCI Only)
• Total Impact factor=205.7
• Avg impact factor=3.02

1. Sidhartha Singh, Navneet Singh, Manoj Baranwal, Siddharth Sharma, Kirthiga Devi S S, Sandeep Kumar (2023). Understanding immune checkpoints and PD-1/PD-L1 mediated immune resistance towards tumor immunotherapy. 3Biotech (accepted and in press) Impact Facor=2.8
2. Heena Kansal, Kranti Garg, Vishal Chopra, Siddharth Sharma. (2023) Role of Thioredoxin in Chronic Obstructive Pulmonary Disease (COPD): A promising future target. Respiratory Research (In press) Impact Factor=5.6
3. Harsh Yadav,  Depanshi Pandit,  Sidhartha Singh,  Parul Sharma,  Kranti Garg,  Nidhi Girdhar,  Karan Sharma,  Vishal Chopra,  Siddharth Chopra,  Siddharth Sharma (2023). GST polymorphism as a predictive biomarker for modulating the susceptibility to chronic obstructive pulmonary disease: A North Indian study. Experimental Physiology. https://doi.org/10.1113/EP091339.
4. Siddharth Sharma Sidhartha Singh, Anu Gupta, Navneet Singh, Parth Sarthi Sengupta, Saroj Kumar Panda, and Siddharth Sharma. (2023). Genotyping, in silico screening and molecular dynamics simulation of SNPs of MGMT and ERCC1 gene in lung cancer patients treated with platinum-based doublet chemotherapy. Journal of biomolecular structure and dynamics doi.org/10.1080/07391102.2023.2261052. (IF=4.4)
5. Jyothish K, Jawahar Lal Joshi, Heena Kansal, Vishal Chopra, Kranti Garg, Nidhi Girdhar, Karan Sharma, Rishabh Deshwal, Siddharth Sharma. (2023). Association of IL4RA and IL-8 polymorphism in predicting susceptibility towards tuberculosis. Monaldi Archives for Chest Disease. https://doi.org/10.4081/monaldi.2023.2594 (IF=1.9)
6. Depanshi Pandit, Parul Sharma, Harsh Yadav, Kranti Garg, Vishal Chopra, Siddharth Sharma. (2023). Association of IL4RA polymorphism in predicting susceptibility towards chronic obstructive pulmonary disease. Pharmacogenomics (Accepted)
7.  Abhishek Choudhary, Adarsh Anand, Amrita Singh, Pratima Roy, Navneet Singh, Vinay Kumar, Siddharth Sharma & Manoj Baranwal. (2023). Machine learning-based ensemble approach in prediction of Lung cancer predisposition using XRCC1 gene polymorphism. Journal of biomolecular structure and dynamics. doi.org/10.1080/07391102.2023.2242492(IF=4.4)
8. Divyanshu Agarwal, SK Singla, Siddharth Sharma. (2023). Effect of rare earth oxide microparticles on mechanical, corrosion, antibacterial, and hemolytic behaviour of Mg-Hydroxyapatite composite for orthopedic applications- a preliminary in-vitro study. Journal of Biomedical Materials Research: Part B - Applied Biomaterials. 111(6) 1232-1246.  IF=3.405
9. Aggarwal, Divyanshu, Kumar, Vinod and Sharma, Siddharth. (2023). Development of Mg-based metal matrix biomedical composites for acicular cruciate ligament fixation by reinforcing with rare earth oxide and hydroxyapatite – A mechanical, corrosion, and microstructural perspective.. Science and Engineering of Composite Materials 30(1) 20220179 https://doi.org/10.1515/secm-2022-0179 (De Gruyter, I.F= 1.9)
10. Parul Sharma, Navneet Singh Siddharth Sharma. (2022). Genetic variations in ABC transporter genes as a predictive biomarker for toxicity in North Indian lung cancer patients undergoing platinum-based doublet chemotherapy. Journal of Biochemical and Molecular Toxicology. 37(3):  e23269. Impact factor=3.568

 

11. Parul Sharma, Navneet Singh Siddharth Sharma. (2022). Impact of ABCB1, ABCC1, ABCC2, and ABCG2 variants in predicting prognosis and clinical outcomes of north Indian lung cancer patients undergoing platinum-based doublet chemotherapy. Journal of Gene Medicine. 2022; 25 (1): e3460. Doi.org/10.1002/jgm.3460  Impact factor=4.152

 

12. Harleen Kaur Walia, Parul Sharma, Navneet Singh, Siddharth Sharma. (2022). Synergistic polymorphic interactions of phase II metabolizing genes and their association toward lung cancer susceptibility in North Indians. International Journal of Environmental Health Research. 17;1-24. (DOI:10.1080/09603123.2022.2133095 in press) Impact factor=4.47
13. Divyanshu Agarwal, Siddharth Sharma, SK Singla. (2022). Investigation of the mechanical and corrosion properties of Mg-Hydroxyapatite composite by addition of rare-earth oxide particulates for biomedical applications. Journal of Mechanical Engineering Science. (237(2):420-439. Impact Factor=1.71      
14. Sidhartha singh, Navneet Singh, Siddharth Sharma (2022). Genetic polymorphisms in the mismatch repair pathway (MMR) genes contribute to hematological and gastrointestinal toxicity in North Indian lung cancer patients treated with platinum‐based chemotherapy. Journal of Biochemical & Molecular Toxicology. 36 (11): e23183 https://doi.org/10.1002/jbt.23183. Impact Factor=3.568      
15. Parul Sharma, Navneet Singh Siddharth Sharma. (2022). Polymorphisms in Solute carrier genes (SLC19A1, SLCO1B1, and SLCO1B3) predicts survival and toxicity in North Indian lung cancer patients undergoing platinum-based doublet chemotherapy. Journal of Clinical Pharmacy and Therapeutics 47(12): 2049-2067  https://doi.org/10.1111/jcpt.13748. Impact Factor=2.15
16. Sidharths singh, Manoj Baranwal, Navneet Singh, Siddharth Sharma (2022). Structural, functional analysis and association of MSH6 rs1800932, rs1042821 polymorphisms with clinical outcome in North Indian lung cancer patients treated with platinum-based doublet chemotherapy. The American Journal of the Medical Sciences. 364(6):735-745  https://doi.org/10.1016/j.amjms.2022.07.005. Impact Factor=3.4
17. Anmol Bhatia, Atul Kumar Upadhyay, Siddharth Sharma. (2022).  miRNAs are now starring in "No Time to Die: overcoming the chemoresistance in cancer. IUBMB LIFE. 75(3):238-256. DOI: 10.1002/iub.2652 2022. Impact Factor= 4.7
18. Harleen Kaur Walia Parul Sharma, Navneet Singh, Siddharth Sharma. (2022). Predictive role of polymorphic variants of phase II drug metabolising enzyme in modulating toxicity in North Indian lung cancer patients undergoing chemotherapy. Xenobiotica. 52(3): 322-331.Impact Factor=1.99
19. Parul Sharma, Siddharth Sharma. (2022). In silico screening and analysis of single-nucleotide polymorphic variants of the ABCC2 gene affecting Dubin–Johnson syndrome. Arab Journal of Gastroenterology.23(3) 172-187 https://doi.org/10.1016/j.ajg.2022.03.003. Impact Factor=1.8
20. Divyanshu Agarwal, SK Singla, Siddharth Sharma. (2022). Drug-Loaded Biomaterials for Orthopedic Applications: A Review. Journal of Controlled Release. 344:113-133. Impact Factor=11.46
21. Shweta Lawanai, Sidhartha Singh, Navneet Singh, Digambar Behera, Siddharth Sharma. (2023). Single Nucleotide Polymorphisms of nucleotide excision repair genes and their association with overall survival in North Indian lung cancer patients treated with platinum-based doublet chemotherapy. Journal of Cancer research & Therapeutics. 19(2):408-419. Impact Factor=1.33
22. Parul Sharma, Navneet Singh, Siddharth Sharma. (2022). Polymorphism in Thymidylate synthase gene predicts survival and toxicity in North Indian lung cancer patients undergoing platinum-based doublet chemotherapy         Oncology. ISSN: 0030-2414 1423-0232 (Online) (DOI:10.1159/000522204) Impact Factor=3.73
23. Harleen Walia, Parul Sharma, Navneet Singh, Siddharth Sharma (2022). Immunotherapy in small cell lung cancer treatment: A promising headway for future perspective. Current treatment options in oncology. 23:268–294. Impact Factor=5.08                                  
24. Nidhi Girdhar, Heena Kansal, Kranti Garg, Siddharth Sharma, K. Sandeep Prabhu, Vishal Chopra, Alexey A. Tinkov, Anatoly V. Skalny, N. Tejo Prakash. (2022). Correlation of serum selenium in asthma patients with severity of the disorder. Biological Trace Element Research. 200(12):4949-4954. https://doi.org/10.1007/s12011-022-03096-8. Impact Factor=4.08
25. Sidhartha Singh, Siddharth Sharma, Manoj Baranwal. (2021). Identification of SNPs in hMSH3/MSH6 interaction domain affecting the structure and function of MSH2 protein        Biotechnology and Applied Biochemistry.  69(6):2454-2465. DOI: 10.1002/bab.2295. Impact Factor=2.72
26. Harleen Kaur Walia, Navneet Singh, Siddharth Sharma. (2021)       Association of NAT-2 gene polymorphisms towards lung cancer susceptibility and prognosis in North Indians. Pharmacogenomics. 23 (2): 97-118. Impact Factor=2.63
27. Sidharths singh, Manoj Baranwal, Navneet Singh, Siddharth Sharma. (2021) Polymorphisms in the MSH2 gene predict poor survival of North Indian lung cancer patients undergoing chemotherapy Biomarkers in Medicine 16(2):69-82. Impact Factor=2.5
28. Harleen Kaur Walia, Navneet Singh, Siddharth Sharma. (2021) GSTP1 Ile105Val polymorphism among North Indian lung cancer patients treated using monotherapy and poly-pharmacy. Human and Experimental Toxicology. 40(12S): S739–S752. Impact Factor=3.247
29. Harleen Kaur Walia, Navneet Singh, Siddharth Sharma. (2021)       MTHFR polymorphism as a predictive biomarker for Gastrointestinal and Hematological toxicity in North Indian adenocarcinoma patients after treatment with pemetrexed and platinum-based doublet chemotherapy. Journal of Chemotherapy. 34(5) 326-340. Impact Factor=2.385
30. Parul Sharma, Navneet Singh, Siddharth Sharma. (2021).  ATP binding cassette (ABC) transporters and cancer: Revisiting the controversial role. Pharmacogenomics. 22(18):1211-1235         Impact Factor=2.63
31. Sidhartha Singh, Navneet Singh, Manoj Baranwal, Siddharth Sharma. (2021). Association of MLH1 -93G>A polymorphisms towards lung cancer susceptibility and its association with clinical outcome in North Indian patients treated with platinum-based chemotherapy. Journal of Cancer research & Therapeutics. Print -0973-1482, Online - 1998-4138. 10.4103/jcrt.jcrt_465_21 Impact Factor=1.33
32. Harleen Kaur Walia, Navneet Singh, Siddharth Sharma. (2021). Genetic Polymorphisms in the microsomal Epoxide Hydrolase (mEH) gene in relation to tobacco smoking: role in lung cancer susceptibility and survival in North Indian Lung cancer patients undergoing platinum-based chemotherapy.        Future Oncology. 17(35): 4925-4946. Impact Factor=3.674
33. Harleen Kaur Walia, Navneet Singh, Siddharth Sharma. (2021). Genetic polymorphism of Arg213His variant in the SULT1A1 gene is associated with reduced susceptibility to lung cancer in North Indian population. Xenobiotica. 51,(9):1071–1080. Impact Factor=1.99
34. Harleen Kaur Walia, Navneet Singh, Siddharth Sharma. (2021). NQO1 polymorphism with clinical outcomes and survival of lung cancer patients treated with platinum chemotherapy. Personalized Medicine. 18(4):333-343. Impact Factor=2.12
35. Ankan Bandyopadhyay, Siddharth Sharma, Digambar Behera, Navneet singh. (2021). UGT1A1 Gene Polymorphisms in Patients with Small Cell Lung Cancer Treated with Irinotecan‐Platinum Doublet Chemotherapy and Their Association with Gastrointestinal Toxicity and Overall survival. The Oncologist. 26(8):701-713. Impact Factor=5.837
36. Anand Manju, Sharma Siddharth, Monga Preetika.(2020). Antimicrobial and antioxidant potential of Tylophora indica (Burm. f.) Merrill and development of an efficient micropropagation system for its therapeutic use Medicinal Plants - International Journal of Phytomedicines and Related Industries. 12(1):82-89.
37. Harleen Kaur Walia, Ushina Verma, Navneet Singh, Siddharth Sharma. (2019). Polymorphisms in GSTM1 and GSTT1 influence response and treatment outcome in lung cancer patients treated with platinum-based chemotherapy.        British Journal of Biomedical Science. 76(4):198-200. Impact Factor=3.829
38. Shweta Lawania, Navneet Singh, Digambar Behera & Siddharth Sharma. (2019).         XPG polymorphisms and their association with lung cancer susceptibility, overall survival and response in North Indian Patients treated with platinum-based doublet chemotherapy. Future oncology. 15(2):151-165 Impact Factor=3.674
39. Amrita singh, Navneet Singh, Digambar Behera & Siddharth Sharma. (2019). XRCC1 632 as a candidate for cancer predisposition via a complex interaction with genetic variants of base excision repair and double strand break repair genes. Future Oncology. 15(33):3845-3859 doi10.2217/fon-2019-0297. Impact Factor=3.674
40. Shweta Lawania, Amrita singh, Navneet Singh, Digambar Behera & Siddharth Sharma. (2019). The multi-faceted high order polymorphic synergistic interactions among nucleotide excision repair genes increase the risk of lung cancer in North Indians. Mutat Res Fund Mol Mech Mutagen. https://doi.org/10.1016/j.mrfmmm.2019.111673. 816-818:111673. Impact Factor=3.151
41. Noorpreet I. Dhanjal, Siddharth Sharma, et al. (2019). Selenium-rich maize modulates the expression of prostaglandin genes in lipopolysaccharide stimulated RAW264.7 macrophages. Food and Function. 10: 2839-2846. Impact Factor=6.317
42. S. Singh, R.S. Kaler and S. Sharma. (2019). FEM simulation analysis of fiber optic surface plasmon resonance sensor based on array of circular gold nanorod. Optik. 183:508-512. Impact Factor=2.84
43. S. Singh, R.S. Kaler and S. Sharma. (2019). Resonance Effect of Bimetallic Diffraction Grating on the Sensing Characteristics of Surface Plasmon Resonance Sensor with COMSOL Multiphysics. Journal of Nanoelectronics and Optoelectronics. 14(5): 669–674. Impact Factor =0.697
44. S. Singh, R.S. Kaler and S. Sharma. (2018). A Novel Two-Dimensional Material Based Optical Fiber Surface Plasmon Resonance Sensor for Sensing of Organic Compounds in Infrared Spectrum Window. Journal of Communications Technology and Electronics. 63: 1269-1275. Impact Factor= 0.529
45. Charu Bahl, Navneet Singh, Digambar Behera, Navneet Singh, Digambar Behera & Siddharth Sharma. (2018). Genetic variants in Wnt antagonist genes predicts the overall survival and prognosis of North Indian lung cancer patients treated with platinum based doublet chemotherapy. Cancer Biotherapy and Radiopharmaceuticals. 33 (10): 466-477. Impact Factor=3.632
46. Shweta Lawania, Navneet Singh, Digambar Behera & Siddharth Sharma. (2018).         Association of XPA Polymorphisms Towards Lung Cancer Susceptibility and its Predictive Role in Overall Survival of North Indians. Biochemical Genetics. 56 (4):375-396. Impact Factor=2.22
47. Amrita Singh, Navneet Singh, Digambar Behera & Siddharth Sharma. (2018). Role of polymorphic XRCC6 (Ku70/ XRCC7 (DNAPKcs) genes towards susceptibility and prognosis of lung cancer patients undergoing platinum based doublet chemotherapy. Molecular Biology Reports. 45(3): 253-261 Impact Factor= 2.742
48. Shweta Lawania, Navneet Singh, Digambar Behera & Siddharth Sharma. (2018).         XPF polymorphism toward lung cancer susceptibility and survival in patients treated with platinum-based chemotherapy. Future Oncology. 14(11): 1071-1089. Impact Factor= 3.674
49. Apurva Bhardawaj, Charu Bahl, Navneet Singh, Digambar Behera & Siddharth Sharma. (2018). Interactive potential of genetic polymorphism in Xenobiotic metabolizing and DNA repair genes for predicting lung cancer predisposition and overall survival in North Indians. Mutation Research and Genetic Toxicology.  826: 15-24. Impact Factor=2.873
50. Amrita Singh, Navneet Singh, Digambar Behera,  Siddharth Sharma. (2018). Genetic Investigation of Polymorphic OGG1 and MUTYH Genes Towards Increased Susceptibility in Lung Adenocarcinoma and its Impact on Overall Survival of Lung Cancer Patients Treated with Platinum Based Chemotherapy. Pathology & Oncology Research. 25: 1327–1340. Impact Factor=2.874
51. Sneha, Charu Bahl, Navneet Singh, Digambar Behera & Siddharth Sharma. (2018).  Role of sequence variations in AhR gene towards modulating smoking induced lung cancer susceptibility and Clinical outcomes in North Indian population: A multiple interaction analysis. Current Genomics.  19: 313-326 Impact Factor=2.689
52. Vijay Kumar Bodal, Shruti Sangwan , Manjit Singh Bal , Mohanvir Kaur , Siddharth Sharma, Bhavleen Kaur. (2017).    Association between Microrna 146a and Microrna 196a2 Genes Polymorphism and Breast Cancer Risk in North Indian Women. Asian Pacific Journal of Cancer Prevention. 18:2345-2348. Impact Factor=2.52
53. Ankita Pandey, Charu Bahl, Navneet Singh, Digambar Behera & Siddharth Sharma. (2017). Functional role of CyclinD1 polymorphism (G870A) in modifying susceptibility and overall survival of North Indian lung cancer patients. Tumori. 104(3): 179-187. Impact Factor= 2.438
54. Charu Bahl, Navneet Singh, Digambar Behera & Siddharth Sharma. (2017). High order gene interactions between the genetic polymorphisms in Wnt and AhR pathway towards lung cancer susceptibility. Personalized Medicine. 14(6): 487-502. Impact Factor=2.12
55. Noorpreet Dhanjal, Siddharth Sharma, Suresh Prabhu, N Tejo Prakash. (2017). Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages. Food and Agricultural Immunology. 28 (6): 1374-1392. Impact Factor=3.268
56. Shweta Lawania, Navneet Singh, Digambar Behera & Siddharth Sharma. (2017). XPD polymorphism towards lung cancer susceptibility survival and response in patients treated with platinum chemotherapy.       Future Oncology. 13(22): 1945–1965. Impact Factor=3.674
57. Charu Bahl, Navneet Singh, Digambar Behera & Siddharth Sharma. (2017). Single Nucleotide Variations in the Wnt antagonist sFRP3 (rs7775 & rs288326) and sFRP4 (rs1802073 &1802074) genes and their association with lung cancer risk in North Indians. Meta Gene. 13: 159-168.
58. Shweta Lawania, Navneet Singh, Digamba rBehera & Siddharth Sharma.       (2017). XPC Polymorphism and risk for lung cancer in North Indian patients treated with platinum based chemotherapy and its association with clinical outcomes. Pathology & Oncology Research. 24(2): 353-366 Impact Factor=2.87
59. Amrita Singh, Navneet Singh, Digambar Behera & Siddharth Sharma. (2017). Polymorphism in XRCC1 gene modulates survival and clinical outcomes of Advanced North Indian Lung cancer patients treated with platinum based doublet chemotherapy. Medical Oncology. 34 (4): 64-78. Impact Factor=3.738
60. Charu Bahl, Navneet Singh, Digambar Behera & Siddharth Sharma. (2017). Association of candidate genetic variations in Axin2 gene with lung cancer risk in North Indian population: A multiple-interaction analysis. Tumor Biology. 39: 1:18. Impact Factor=3.65
61. Amrita Singh, Navneet Singh, Digambar Behera & Siddharth Sharma. (2016). Association and multiple interaction analysis among five XRCC1 polymorphic variants in modulating lung cancer risk in North Indian population. DNA Repair. 47: 30–41. Impact Factor=4.354
62. Charu Bahl, Navneet Singh, Digambar Behera & Siddharth Sharma. (2016). Association of polymorphisms in Dickopff (DKK) gene towards modulating risk for lung cancer in north Indians. Future Oncology. 13 (3): 213-232. Impact Factor=3.674
63. Ankita Kumar, Charu Bahl, Navneet Singh, Digambar Behera & Siddharth Sharma. (2016). Association of p53 codon 72 polymorphism and survival of North Indian lung cancer patients treated with platinum-based chemotherapy. Molecular Biology Reports. 43: 1383–1394. Impact Factor=2.742
64. Yashila Girdhar, Navneet Singh, Digambar Behera & Siddharth Sharma. (2016). Synergistic association of CYP1A1 polymorphisms with increased susceptibility to squamous cell lung cancer in north Indian smokers. Int J Biol Markers. 31(4):e402-e412. Impact Factor=3.248
65. Girdhar Y, Singh N, Behera D, Siddharth Sharma. (2016). Combinations of the variant genotypes of CYP1A1, GSTM1 and GSTT1 are associated with an increased lung cancer risk in North Indian population: a case-control study. Pathology & Oncology Research. 22(3): 647-652. Impact Factor=2.87
66. Sharma N, Singh A, Singh N, Behera D, Siddharth Sharma. (2015). Genetic polymorphisms in GSTM1, GSTT1 and GSTP1 genes and risk of lung cancer in a North Indian population. Cancer Epidemiology. 39(6): 947-955.  Impact Factor=2.89
67. Sodhi KK, Bahl C, Singh N, Behera D, Siddharth Sharma. (2015). Functional Genetic Variants in pre-miR-146a and 196a2 genes are associated with risk of lung cancer in North Indians. Future Oncology. (15):2159-2173. Impact Factor=3.674
68. Noorpreet I. Dhanjal, Siddharth Sharma, and N. Tejo Prakash. (2016). Quantification and In Vitro Bioaccessibility of Selenium from Osborne Fractions of Selenium-Rich Cereal Grains. Cereal Chemistry. 93 (4): 339-343. Impact Factor=2.534
69. Saronika Jindal, Shruti Sobti; Mukesh Kumar; Siddharth Sharma; Manoj Kumar Pal. (2016) Nanocavity-Coupled Photonic Crystal Waveguide as Highly Sensitive Platform for Cancer Detection. IEEE Sensors Journal. (10): 3705-3710. Impact Factor=4.325
70. Siddharth Sharma, Shariatmadar Sherry;  Krishan Awtar. (2010).        Electronic Volume, Aldehyde dehydrogenase and Stem Cell Marker Expression in Cells from Human Peripheral Blood Apheresis Samples. Cytometry B (Clinical Cytometry). 78B           :123-129. Impact Factor=3.248
71. Krishan Awtar, Deepti Sharma, Siddharth Sharma, (2010). ALDH+/CD44+/CD24- Expression in Cells from Body Cavity Fluids.  Clinical Cytometry (Cytometry B). 78B: 176-182. Impact Factor=3.248
72. Siddharth Sharma; Cabana Raquel , Shariatmadar Sherry;  Krishan Awtar. (2008).  Cellular volume and marker expression in human peripheral blood apheresis stem cells.           Cytometry Part A. 73 (2): 160-167. Impact Factor=4.714
73. Shariatmadar Sherry;  Siddharth Sharma. (2008). Electronic volume of CD34 positive cells from peripheral blood apheresis samples. Cytometry. Part B, Clinical Cytometry. 74 (3): 182-188 Impact Factor=3.248
74. S. Agarwal*, S. Sharma*, V. Agarwal and N. Roy*(2005).        Caloric Restriction Augments ROS Defense in S. cerevisiae, by a Sir2p Independent Mechanism. Free Radical Research. 39:55-62. Impact Factor=4.354
75. R.C. Sobti,*, A.I. Al-Badran, S. Sharma, S.K. Sharma, A. Krishan, H. Mohan. (2005).  Genetic polymorphisms of CYP2D6, GSTM1, and GSTT1 genes and bladder cancer risk in North India. Cancer Genetics and Cytogenetics. 156: 68–73. Impact Factor=2.169
76. Sobti, R.C, Sharma, S, Jindal; 5K., and Janmeja; A. (2004). Genetic polymorphism of the CYP1A1, CYP2ET, GSTMI and GSTTI genes and lung cancer susceptibility in North Indian population. Molecular & Cellular Biochemistry            . 266: 1-9. Impact Factor=3.842
77. Sobti, R.C, Sharma, S, Joshi, A., .Jindal, S.K., Janmeja, A. (2003). CYPIAI and CYP2D6 polymorphism and risk towards lung cancer in a North Indian population. Biomarkers. 8(5): 415-428. Impact Factor=2.66
78. Gill, T., Sharma, V., Mamtesh, Sharma, S., Sobti, R.C. (2003). RAPD-PCR analysis in two species of Catopsilia. Carologia. 56: 100-105. Impact Factor=1.47
79. RC Sobti, S Sharma (2001). Molecular genetics of cancer with special reference to cancers of GI tract, Lung, Head and Neck and Cervix. JPAS. 1: 63-67.
80. RC Sobti, S Sharma (2000). Molecular markers in lung cancer. Nucleus. 43(1,2): 71-82.         

 

Research Projects

Details of Projects completed during the last 5 years

S. No	Title	Cost in Lakh	Duration	Role as PI/Co-PI	Agency
1	Association of single nucleotide polymorphisms and methylation status in the wnt and ahr signaling pathways with risk for occurrence of lung cancer in North Indians	27.5	2012-2015	PI	I.C.M.R, New Delhi
2	Studies on anti-inflammatory responses as function of bioavailable selenium from Se-rich cereal grains	19.92	2012-2015	Co-PI	C.S.I.R, New Delhi

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Book Chapters:

 

1.  Siddharth Sharma and Awtar Krishan. Electronic Volume of Hematopoietic Stem Cells. In:Flow Cytometry Applications in Stem Cell Research, Eds. Awtar Krishan, H Krishnamurthy and Satish Totey, John Wiley-Blackwell Publications; 2010.

 

2. Molecular genetics of Gastrointestinal Tract Cancers. (2002). In book:Some aspects of Chromosome Structure and Function. Pp 177-1 92 (Eds) Sobti, R.C. eta!. Publishers (Kluwer & Narosa).

 

3. Telomere Telomerase and Cancer. (2002), In book: Some aspects of Chromosome Structure and Function. pp 168-191 (Eds) Sobti, R.C. et at Publishers (Kluwer & Narosa).

 

4.     Molecular pathogenesis of lung cancer (2002). In book: Some aspects of Chromosome

Structure and Function. pp 193-205 (Eds) Sobti, R.C. et at Publishers (Kluwer & Narosa).

 

 

 

Membership of national/International organization

1. Member of clinical cytometry society (USA)

 

Honors/Awards:

1. Awarded Young Scientist Award from Panjab Academy of Sciences 2002

2. Was awarded with the “Best Research Paper” in the year 2019 for the paper titled “Functional genetic variants in pre-miR-146a and 196a2 genes are associated with risk of lung cancer in North Indians” by Thapar Institute of Engineering & Technology, Patiala

3. Awarded Performance Incentive Scheme for the year 2014 by Thapar Institute of Engineering & Technology, Patiala

4. Post-Doctoral fellowship award by Procognia Ltd (2005-2006)

5. Post-Doctoral fellowship award by Miller school of Medicine, (2007-2009)

POSTER PRESENTATIONS/ORAL PRESENTATIONS/ABSTRACTS:

1. XPD polymorphism and its association with lung cancer risk, overall survival and response in north Indian patients treated with platinum based chemotherapy. S. Lawania, S. Sharma, N. Singh, D. Behera. Poster presentation at 2nd Indian cancer congress 08 - 12 November, 2017  Bengaluru,
2. Role of Polymorphic Variants of BER and DSBR Pathway Genes in Modulating Lung Cancer Susceptibility and Prognosis of North Indian Population” A. Singh, S. Sharma, N. Singh, D. Behera. Presented at IASLC 18TH WORLD CONFERENCE ON LUNG CANCER, October 15-18, 2017, Yokohama, Japan.
3. UGT1A1 gene polymorphisms and clinical outcomes in small cell lung cancer (SCLC) patients treated with irinotecan-platinum chemotherapy: The first study from north India. Navneet Singh, Ankan Bandyopadhyay, Siddharth Sharma, Digambar Behera. Abstract presentation. 2017 American Association of clinical oncology (ASCO) Annual Meeting (June 2-6, 2017) Chicago, Illinois.
4. Genetic variants in the Wnt antagonist genes (sFRP, Dkk and Axin2) predicts the overall survival and prognosis of North Indian lung cancer patients treated with platinum based doublet chemotherapy. Charu Bahl, Siddharth Sharma, Navneet Singh, Digambar Behera. Poster Presentation at CHASCON 2017 meeting9-11 March, 2017, Panjab University, Chandigarh
5. Se-dietary matrices can upregulate the anti-inflammatory responses in RAW macrophages. Noorpreet Dhanjal Siddharth Sharma, Sandeep Prabhu Kumble, Tejo Prakash Nagaraja. Poster Presentation at 4th International Conference on Selenium in the Environment and Human Health. (18-21 October 2015, Sao Paulo, Brazil)
6. Synergistic association of CHRNA5 (rs16969968) polymorphism towards increased susceptibility to squamous Cell Lung Cancer in North Indian Smokers. Yashila Girdhar, Siddharth Sharma, Navneet Singh, Digambar Behera. Oral Presentation at International Conference on Advances in Biomedical Engineering, cancer Biology, bioinformatics and applied biotechnology (ABECBAB) 25-26th July 2015, JNU, New Delhi.
7. CyclinD1 polymorphism (G870A) in modifying susceptibility and overall survival of North Indian lung cancer patients. Ankita Pandey, Siddharth Sharma, Navneet Singh, Digambar Behera. Oral Presentation at International Conference on Advances in Biomedical Engineering, cancer Biology, bioinformatics and applied biotechnology (ABECBAB) 25-26th July 2015, JNU, New Delhi.
8. Stem cell marker expression in cells from body cavity fluids. Awtar Krishan, Deepati Sharma, Siddharth Sharma, Ronald Hamelik, Parvin Ganjei-Azar, and Mehrdad Nadji. Presented at 24th Annual Meeting of the Clinical Cytometry Society October 16–18, 2009, Jacksonville, Florida, USA.
9. Electronic Volume and ALDH expression in Stem cells from human Apheresis samples by Siddharth Sharma, Sherry Shariatmadar, Raquel Cabana, and Awtar Krishan. Presented at The 23rd Annual Meeting of the Clinical Cytometry Society October 12–14, 2008 Portland, Oregon, USA
10. Electronic  Volume  of  Stem  Cells  in  Human  Apheresis  Samples  by  Siddharth Sharma, Raquel Cabana, Sherry Shariatmadar, Awtar Krishan. Presented at the ISAC XXIV International Congress, May 17-21, 2008 held at Budapest Hungary
11. Electronic Cell Volume and Marker Expression in Stem Cells from Human Apheresis Samples by Siddharth Sharma, Raquel Cabana, Sherry Shariatmadar, Awtar Krishan. Presented at the AACR at San Diego, CA, USA on April12-16 2008.
12. Electronic  Volume  of  Hematopoietic  Stem  Cells  by  Siddharth  Sharma,  Raquel Cabana, Sherry Shariatmadar, Awtar Krishan. Presented at the 22nd  Annual Clinical Cytometry society at Washington D.C. on October 16-19 2007.
13. “P450-BIOCHIPS by Siddharth Sharma Natasha Beeton and JM Blackburn. Presented at the international conference on “A Molecular Meander in the Midlands” organized by South African Society for Biochemistry and Molecular Biology at PeiterMaritzburg, South Africa, on July 3-56h, 2006.

INVITED LECTURE

• Guest lecture on “Flow cytometry and its role in modern biology” delivered to department of biotechnology GGDSD College, Chandigarh 11th of November 2017.
• Guest speaker on “Flow cytometry and its clinical applications” in 49th Annual Conference of Indian Pharmacological Society (IPSCON-2016), India Scheduled on 20th to 23rd October 2016.

LIST OF COMPLETED DOCTORAL THESIS (Ph.D/DM)

1. Ms Charul Bahl: “Study of the Genetic polymorphisms and Methylation profile in the Wnt signaling pathway with risk of occurrence of Lung cancer in North Indian population”
2. Ms Amrita Singh: “Single Nucleotide Polymorphisms in the Base Excision Repair and Double Strand Break Repair pathway genes in Lung Cancer patients and their correlation with clinical outcomes”
3. Ms Shweta Lawania: “Studies on genetic polymorphism in nucleotide excision repair genes in relation to lung cancer patients”
4. Ms Harleen Kaur Walia: “The role of Phase II detoxification genes towards modulating risk for lung cancer and its association with clinical outcomes”
5. Mr Sidhartha Singh: “Single nucleotide polymorphism in mismatch repair     pathway genes, and its computational analysis in lung Cancer patients”
6. Ms Noorpreet Dhanjal: “Anti-inflammatory responses of bioavailable selenium from selenium-rich cereal grains”
7. Mr Sarabjit Singh: “Investigation of Fibre Optic Sensor based on Surface Plasmon Resonance for Sensing Applications”
8. Dr Ankan Bandopadhay: “UGT1A1 polymorphisms in small cell lung cancer patients undergoing irinotecan-based chemotherapy and their association with clinical outcomes”

 

LIST OF COMPLETED MD THESIS (MD)

1. Dr Nidhi Girdhar: “Assessment of serum levels of selenium and the role of il-4/il4ra polymorphism in patients of asthma and its correlation with severity of asthma”

 

2. Dr Harsh Yadav: “Role of GSTM1 and GSTT1 as a risk factor for development of chronic obstructive pulmonary disease”

 

 

             LIST OF COMPLETED M.TECH THESIS

 

1. Neha Sharma: “Genetic Polymorphism in the Phase II (GSTM1 & GSTT1 & GSTP1) Detoxification Genes with Risk for Occurrence Lung Cancer and Its Association With Clinicopathological Features In North Indian Population”
2. Ms Kushal Deep Sodhi: “Association of single nucleotide polymorphism in microrna146a and 196a2 gene towards risk for lung cancer in North indian population”
3. Ms Sneha Budhwar: “Aryl hydrocarbon Receptor gene polymorphism and its association towards Lung Cancer risk and Clinical outcomes”
4. Ms Shweta Parmar: “Phytochemical Studies and Evaluation of Antimicrobial Potential of Native and Micropropagated Plants of Tylophora indica: A Comparative Study”
5. Ms Ankita Mukherjee: “Prognostic role of p53 codon 72 polymorphism in North Indian Lung cancer patients”
6. Ms Sheetal Vats: “Role of TGF-β and Wnt antagonist gene sFRP4 in predicting overall survival and clinic-pathological outcomes in lung cancer patients treated with platinum based doublet chemotherapy”
7. Ms Yashila Girdhar: “Synergistic association of CYP1A1 polymorphisms with increased susceptibility to squamous cell lung cancer in north Indian smokers”
8. Ms Ankita Panday: “Cyclin d1(ccnd1 gene) and its polymorphism in cancer”
9. Ms Sonika Chib: “Search for Novel SNPs in XPC, XPD and XPG Genes”
10. Ms Anu Gupta: “Prognostic significance of MGMT & ERCC1 gene polymorphism in lung cancer patients treated with platinum based doublet chemotherapy”
11. Ms Shivani Mathur: “Quality Assurance and Regulatory process for Orthopedics”
12. Ms Parampreet Kaur: “Identification and characterization of functional single nucleotide polymorphisms (SNPs) in the destruction complex of Wnt signalling pathway involved in lung carcinogenesis: A computational approach”
13. Ms Deepanshi Pandit: “Impact of GSTM1, GSTT1, and IL-4 R(α) genetic polymorphisms toward susceptibility for Chronic Obstructive Pulmonary Disease (COPD)”

 

LIST OF COMPLETED MSc THESIS

1. Ms Kamaldeep Kaur: “SNP genotyping of RECK gene in lung cancer”
2. Ms Nerrittee: “Genetic variation in CYP1A1 gene towards risk of occurrence of lung carcinoma in north Indian population”
3. Ms Poonam Naik: “Role of XRCC1 DNA Repair Gene Polymorphisms (Arg399Gln and Arg194Trp) on Outcome of Platinum Based Chemotherapy for Non-Small Cell Lung Cancer in North Indian Population”
4. Mr. Rishi Roy:” Association of Micro RNA Gene 196a2 and 146a Polymorhism towards Risk for Breast Cancer”
5. Ms Rashmi Pal: “Case-control study of HER2/ neu Ile655Val single nucleotide polymorphism in relation towards risk for breast cancer”
6. Ms Apurva Bhardawaj: “Association of Aryl Hydrocarbon Receptor gene polymorphism with the risk of Lung Cancer in North Indian population”
7. Mr Ajit Mourya: “FOXP3 rs2294021 Polymorphism Towards the Susceptibility To Breast Carcinoma In North Indian Population”
8. Ms Anjali Rani: “Cytotoxic Potential of fungal extracts against A549 cell line and its potential in drug development”
9. Ms Harindar Kaur: “Association of rs2073498 ala133ser gene polymorphism with risk towards lung cancer susceptibility in a north Indian population”
10. Ms Inderjot Kaur: “Association of single nucleotide polymorphism in microrna 196a2 and 146a gene towards risk for lung cancer in north Indian population”           
11. Ms Preetika Monga: “Evaluation of Antimicrobial and Antioxidant potential of native and micropropagated plants of Tylophora indica”
12. Ms Divya Ahluwalia: “Genetic Polymorphism in NQO1 gene and its Association with  Lung Cancer  in North Indian Population”
13. Ms Isha Sharma: “In silico analysis of SNPs in ABCB1 gene affecting lung cancer associated protein P – glycoprotein (P-gp)”
14. Ms Ishita Sharma: “Decoding the significance of toll-like receptor (TLR) signaling in cerebral malaria pathology”
15. Ms Kirti Rai: “Prognostic significance of ABCB1 (MDR-1) gene variants (C1236T and G2677T) in lung cancer patients treated with platinum-based doublet chemotherapy”
16. Ms Manpreet Kaur: “Role of DNMT3B genetic polymorphism (rs1569686) in predicting overall survival and clinic-pathological outcomes in lung cancer patients treated with platinum based doublet chemotherapy”
17. Ms Neha Garg: “In silico analysis of single nucleotide polymorphisms of XPD gene in lung cancer patients”
18. Ms Nitika: “Identification of functional Single Nucleotide Polymorphisms (SNPs) in Superoxide Dismutase 3 (SOD3) gene and its association with Chronic Obstructive Pulmonary Disease (COPD)”
19. Ms Prerna Bhagat: “GSTP1 as a potential predictive factor for adverse events associated with platinum-based antitumor agent-induced toxicity”
20. Ms Heena Kansal: “Polymorphisms in the IL4, IL4Ra genes and its synergistic association with selenium status in patients with asthma severity”
21. Ms Poloumi Chandra: “Cloning, Overexpression and Purification of RNase D in Escherichia coli”
22. Ms Prabhjot Kaur: “Genetic Polymorphism of Thymidylate Synthase gene in Lung Cancer Patients Undergoing Platinum Based Chemotherapy”
23. Ms Shitanshu Sharma: “Polymorphism in The UGT1A1 Gene and its Association with Small Cell Lung Cancer in North Indian Population”
24. Ms Ushina Verma: “Predictive potential role of GSTT1 and GSTM1 genes in the overall survival and treatment outcome of lung cancer patients treated with platinum-based doublet chemotherapy”
25. Ms Anindita Saha: “Assessment of Optimal Conditions for L-asparaginase Production by Endophytic Lasiodiplodia Pseudothreobromae”
26. Ms Shweta Parmar: “Phytochemical Studies and Evaluation of Antimicrobial Potential of Native and Micropropagated Plants of Tylophora indica: A Comparative Study”

 

EXPERT REVIEWER FOR REPUTED JOURNALS

• Discovery Oncology
• Scientific Reports
• World Surgical Oncology
• Genetic & Molecular Testing
• Future Oncology
• Pharmacogenomics
• Journal Cancer Management and Research
• OncoTargets and Therapy  
• Cancer Biotherapy and Radiopharmaceuticals
• Archives of Medical Research
• Journal of Clinical Laboratory Analysis
• BMC Cancer
• Heliyon
• Gene Reports
• Disease Markers
• Future Oncology
• Cancer Genetics
• Pulmonary Pharmacology