Dr. N Das

Professor

Specialization

Plant Molecular Biology, Genetic Engineering

Email

ndas@thapar.edu; Date of Joining: 22-02-2000

Specialization

Plant Molecular Biology, Genetic Engineering

Email

ndas@thapar.edu; Date of Joining: 22-02-2000

Education

  1. Ph.D., University of Calcutta (1996), Cloning, sequencing and characterization of seed-expressed acyl carrier protein (ACP) genes from Brassica campestris (Agrani), and studies of their expression during seed development (Ph.D. thesis work was carried out at Bose Institute, Kolkata)
  2. M.Sc University of Calcutta (1984)
  3. B.Sc. Presidency College, currently known as Presidency University, Kolkata (1982)

Experience

Teaching Experience:      24 years

Research Experience:       29 years

No. of SCI Publications:   30

Teaching Interests

Recombinant DNA Technology, Metabolic Engineering, Molecular Farming, Cell and Molecular Biology, Cell Biology, Protein Engineering and Enzyme Technology, Plant Biotechnology, Biochemistry, Enzymology 

Research Interests

Biochemical and molecular studies on different genes involved in carbohydrate metabolism particularly with regard to cold-induced sweetening in the potato (Solanum tuberosum L.) tubers, Inhibition of gene functions in potato through transgenics, Isolation and functional characterization of tuber-specific gene promoters, Genetic diversity of bio-diesel crop (Jatropha curcas L.), Nanobiotechnology

Achievements, Awards & Recognitions:

  1. Received a short-term Fellowship from FAO of the United Nations, and worked in the area of plant molecular biology at Calgene Inc, Davis, California, USA (1992).
  2. Postdoctoral Research in the area of reproductive molecular biology at University of Kansas Medical Center, Kansas City, USA (1995-1997)
  3. Head, Department of Biotechnology & Env. Sciences, TIET (May 2005 to Nov 2010)
  4. Worked as Chairman/Member, Board of Studies on Biotechnology, Punjab Technical University (PTU) Jalandhar
  5. Examiner of Ph.D. and M.Tech. theses of other universities/institutions
  6. Participated and contributed in a number of Conferences/Symposia
  7. Delivered lectures in the universities/colleges/conferences/various training courses
  8. Reviewer of a number of peer-reviewed international journals

Publications in the SCI/Impact Factor Journals

Review Paper

Gurpreet Kaur, Sahil Jain, Sakshi Bhushan, Niranjan Das, Munish Sharma, Deepak Sharma (2024) Role of microRNAs and their putative mechanism in regulating potato (Solanum tuberosum L.) life cycle and response to various environmental stresses. Plant Physiology and Biochemistry207, 108334 https://doi.org/10.1016/j.plaphy.2024.108334

Yadveer Kaur, Niranjan Das (2022) Roles of Polyamines in Growth and Development of the Solanaceous Crops Under Normal and Stressful Conditions. Journal of Plant Growth Regulation.  https://doi.org/10.1007/s00344-022-10841-9

Research Articles

Yadveer Kaur,  Niranjan Das (2023) Molecular, in silico and expression studies on lipoxygenases (LOXs) in potato (Solanum tuberosum L.). 3 Biotech 13 (12), 419   

https://doi.org/10.1007/s13205-023-03839-x

Gurpreet Kaur, Niranjan Das (2023) Fructokinases in potato (Solanum tuberosum L.): molecular cloning, sequence analyses, three-dimensional structure prediction, enzyme activity and purification by aqueous two-phase system. Journal of Plant Biochemistry and. Biotechnololgy 32, 632–646.

Yadveer Kaur, Niranjan Das (2023) Gibberellin 2-Oxidases in Potato (Solanum tuberosum L.): Cloning, Characterization, In Silico Analysis and Molecular Docking. Molecular Biotechnology

 https://doi.org/10.1007/s12033-023-00745-8

Shreya Sharma, Niranjan Das, Bonamali Pal (2023) Preparation, characterization, and application of soy protein isolate/Mg–Al layered double hydroxide-based bionanocomposite films. Chemical Papers 77, 3265–3275

Gurpreet Kaur, Niranjan Das (2022) An isoform of sucrose synthase involved in sink strength of potato (Solanum tuberosum L.): Molecular cloning, sequence analyses, 3-D structure, crucial motifs and expression. South African Journal of Botany 149: 446-457
Gurpreet Kaur, Niranjan Das (2022) Molecular cloning, expression and in silico analyses of calcium˗dependent protein kinase 2 (CDPK2) in potato (Solanum tuberosum L.). South African Journal of Botany 148: 634-642
Guriqbal Singh Dhillon, Niranjan Das, Satinder Kaur, Puja Srivastava, Navtej Singh Bains, Parveen Chhuneja (2021) Marker assisted mobilization of heat tolerance QTLs from Triticum durum-Aegilops speltoides introgression lines to hexaploid wheat. Indian Journal of Genetics (The Indian Society of Genetics & Plant Breeding) 81(2): 186-198
Guriqbal Singh Dhillon, Satinder Kaur, Niranjan Das, Rohtas Singh, Jesse Poland, Jaspal Kaur Parveen Chhuneja (2020) QTL mapping for stripe rust and powdery mildew resistance in Triticum durumAegilops speltoides backcross introgression lines. Plant Genetic Resources: Characterization and Utilization 18 (4): 211-221
Gurpreet Kaur, Shweta Sharma, Niranjan Das (2020) Comparison of catalase activity in different organs of the potato (Solanum tuberosum L.) cultivars grown under field condition and purification by three‑phase partitioning. Acta Physiologiae Plantarum 42: 10 (P1- P11).
Rajneesh Kumar, Niranjan Das (2018) Seed oil  of  Jatropha curcas L. germplasm: Analysis of oil quality and fatty acid composition. Industrial Crops & Products 124: (2018) 663–668
Rajneesh Kumar, Niranjan Das (2018) Survey and selection of Jatropha curcas L. germplasm: assessment of genetic variability and divergence studies on the seed traits and oil content. Industrial Crops & Products 118:125–130
Rayees Ahmad Rather, Ramandeep K. Sarwara, Niranjan Das, Bonamali Pal (2018) Impact of reducing and capping agents on carbohydrates for the growth of Ag and Cu nanostructures and their antibacterial activity. Particuology,  https://doi.org/10.1016/j.partic.2018.01.004
Samriti Thakur, Kirandeep Kaur, Niranjan Das, Bonamali Pal (2017) Photo-oxidation kinetics of sugars having different molecular size and glycosidic linkages for their complete mineralization to subunits by bare/Ag–TiO2 under UV irradiation. Journal of the Taiwan Institute of Chemical Engineers 80: 488–494
Tanushree Basu, Khyati Rana, Niranjan Das, Bonamali Pal (2017) Selective detection of Mg2+ ions via enhanced fluorescence emission using Au–DNA nanocomposites. Beilstein Journal of Nanotechnology 8: 762–771
Raghavendra Aminedi, Niranjan Das (2014) Class I patatin genes from potato (Solanum tuberosum L.) cultivars: molecular cloning, sequence comparison, prediction of diverse cis-regulatory motifs, and assessment of the promoter activities under field and in vitro conditions. In Vitro Cellular & Developmental Biology-Plant 50:673–687
Dhakshi Taneja, Niranjan Das (2014) Molecular cloning, sequence analyses, and expression studies of sucrose-phosphate synthase in the potato (Solanum tuberosum L.) cultivars. Acta Physiologiae Plantarum 36: 2253–2269
Vijay Kumari, Niranjan Das (2013) Vacuolar invertases in potato (Solanum tuberosum L.): molecular cloning, characterization, sequence comparison, and analysis of gene expression in the cultivars. Acta Physiologiae Plantarum 35: 2055–2068
Anshu Bansal, Niranjan Das (2013) Molecular cloning and sequence comparison of a cDNA encoding α-glucan, water dikinase (GWD) from potato (Solanum tuberosum L.), and analysis of gene expression. Journal of Plant Biochemistry and Biotechnology 22 (4): 441–452
Raghavendra Aminedi, Gunveen Wadhwa, Niranjan Das, Bonamali Pal (2013) Shape-dependent bactericidal activity of TiO2 for the killing of Gram-negative bacteria Agrobacterium tumefaciens under UV torch irradiation. Environmental Science and Pollution Research 20: 6521–6530
Anshu Bansal, Vijay Kumari, Dhakshi Taneja, Rupinder Sayal, Niranjan Das (2012) Molecular cloning and characterization of granule-bound starch synthase I (GBSSI) alleles from potato and sequence analysis for detection of cis-regulatory motifs. Plant Cell, Tissue and Organ Culture (PCTOC) 109: 247–261
Bonamali Pal, Isha Singh, Kunal Angrish, Raghavendra Aminedi, Niranjan Das (2012) Rapid photokilling of gram-negative Escherichia coli bacteria by platinum dispersed titania nanocomposite films. Materials Chemistry and Physics 136: 21–27
Vijay Kumari, Anshu Bansal, Raghavendra Aminedi, Dhakshi Taneja, Niranjan Das (2012) Simplified extraction of good quality genomic DNA from a variety of plant materials. African Journal of Biotechnology 11(24):6420–6427
Hirock Jyoti Datta, P Mandal, R. Bhattacharya, Niranjan Das, Subrata Sau, N C Mandal (2007) The G23 and G25 genes of temperate Mycobacterio-phage L1 are essential for the transcription of its late genes. Journal of Biochemistry and Molecular Biology 40:156–162
Indrani Datta, Sarbani Banik-Maiti, L Adhikari, Subrata Sau, Niranjan Das, N. C Mandal (2005) The mutation that makes Escherichia coli resistant to  P gene-mediated host lethality is located within the DNA initiator gene dnaA of the bacterium. Journal of Biochemistry and Molecular Biology 38:89–96
Paria B C, Das Niranjan, Das S K, Zhao X, Dileepan K N, Dey S K (1998) Histidine decarboxylase gene in the mouse uterus is regulated by progesterone and correlates with uterine differentiation for blastocyst implantation. Endocrinology 139:3958–3966
Niranjan Das, Jue Wang, Sudhansu K. Dey (1997) Uterine preparation for implantation in the mouse is associated with coordinate expression of estrogen-responsive finger protein and estrogen receptor. Molecular Reproduction and Development 46:499–506
S.K. Das, Niranjan Das, J. Wang, H. Lim, B. Schryver, G.D. Plowman, S.K. Dey (1997) Expression of betacellulin and epiregulin genes in the mouse uterus temporally by the blastocyst solely at the site of its apposition is coincident with the window of implantation. Developmental Biology 190:178–190
Niranjan Das, Sudhamoy Ghosh. (1995) Molecular cloning and sequence analysis of a seed-expressed acyl carrier protein (ACP) gene from Brassica campestris (Agrani). Biochimica et Biophysica Acta (BBA Lipids and Lipid Metabolism) 1258:75–79

 

Details of GenBank (NCBI) Submissions 

  • ON804239   Solanum tuberosum cultivar Kufri Chipsona-1 transcription factor POTM1-1A (POTM1-1) mRNA, complete cds  821 bp    mRNA   Submitted (18-JUN-2022)  AUTHORS: Kaur, Y., Kaur, R. and Das, N.     

  • ON804240    Solanum tuberosum cultivar Kufri Chipsona-1 gibberellin 2-oxidase 1 (GA2ox1) mRNA, complete cds  1105 bp  mRNA Submitted (18-JUN-2022) AUTHORS: Kaur, Y. and Das, N.

  • MT748043 Jatropha curcas curcin 2A mRNA, complete cds 947 bp    mRNA. Submitted (10-JUL-2020)  AUTHORS: Bhardwaj, N., Kaur, G., Kumar, N., Kumar, R. and Das, N.

  • MT731684 Solanum tuberosum sucrose synthase (SUS) mRNA, complete cds  2668 bp  mRNA Submitted (07-JUL-2020)  AUTHORS: Kaur, G. and Das, N.

  • MT731685 Solanum tuberosum catalase (CAT1) mRNA, complete cds 1600 bp    mRNA Submitted (07-JUL-2020)  AUTHORS: Kaur, G. and Das, N.

  • MN420513 Solanum tuberosum fructokinase (FRK) mRNA, FRK-2 allele, complete cds  1110 bp    mRNA   Submitted (06-SEP-2019) AUTHORS: Kaur, G. and Das, N.

  • MN420514 Solanum tuberosum calcium-dependent protein kinase 2 (CDPK2) mRNA, CDPK2-A allele, complete cds     1560 bp   mRNA Submitted (06-SEP-2019)  AUTHORS: Kaur, G. and Das, N.

  • MN401256 Solanum tuberosum cultivar Kufri Chipsona-1 fructokinase (FRK2) mRNA, FRK2A allele, partial cds     784 bp    mRNA Submitted (30-AUG-2019)  AUTHORS: Kaur, G. and Das, N.

  • MN401257 Solanum tuberosum cultivar Kufri Chipsona-1 fructokinase (FRK2) mRNA, FRK2B allele, partial cds  784 bp    mRNA Submitted (30-AUG-2019)  AUTHORS: Kaur, G. and Das, N.

  • MN401258 Solanum tuberosum cultivar Kufri Chipsona-1 fructokinase-like protein (FLN1) mRNA, complete cds      839 bp    mRNA  Submitted (30-AUG-2019)  AUTHORS: Kaur, G. and Das, N.

  • JX124230 Solanum tuberosum cultivar Kufri Chandramukhi clone PM01 class I patatin gene, promoter region and partial cds  1454 bp  DNA  Submitted (02-JUN-2012)  AUTHORS: Aminedi, R. and Das, N.     

  • JX124231  Solanum tuberosum cultivar Kufri Chandramukhi clone PM02 class I patatin gene, promoter region and partial cds  1441 bp    DNA  Submitted (02-JUN-2012)  AUTHORS: Aminedi, R. and Das, N.

  • JX124227  Solanum tuberosum cultivar Kufri Jyoti clone PN02 class I patatin gene, promoter region and partial cds  2747 bp    DNA  Submitted (29-MAY-2012)  AUTHORS: Aminedi, R., Gupta, M. and Das, N.  

  • JX124228  Solanum tuberosum cultivar Kufri Chipsona 1 clone PK01 class I patatin gene, promoter region and partial cds    2332 bp    DNA  Submitted (30 MAY-2012) AUTHORS: Aminedi, R., Gupta, M. and Das, N.     

  • JX124229  Solanum tuberosum cultivar Kufri Chipsona 1 clone PK03 class I patatin gene, promoter region and  partial cds  874 bp    DNA  Submitted (30-MAY-2012)      AUTHORS: Aminedi, R., Gupta, M. and Das, N.

  • EU684230  Solanum tuberosum sucrose-phosphate-synthase (sps) mRNA, complete cds  3591 bp    mRNA Submitted (29-APR-2008)  AUTHORS: Taneja, D., Bansal, S. and Das, N.

  • EU622806  Solanum tuberosum clone AI-01 beta-fructofuranosidase mRNA, partial cds  2013 bp  mRNA Submitted (05-APR-2008) AUTHORS: Kumari, V. and Das, N.

  • EU622807  Solanum tuberosum clone AI-02 beta-fructofuranosidase mRNA, complete cds  1945 bp  mRNA  Submitted (05-APR-2008)  AUTHORS: Kumari, V. and Das, N.

  • EU599037  Solanum tuberosum starch-granule-bound R1 protein (R1) mRNA, complete cds 4789 bp    mRNA    Submitted (29-MAR-2008)  AUTHORS: Bansal, A. and Das, N.

  • EU548081  Solanum tuberosum clone GKCM-TU01 granule-bound starch synthase (waxy) gene, partial cds     1320 bp    DNA   Submitted (01-MAR-2008)  AUTHORS: Bansal, A., Kumari, V., Sayal, R. and Das, N.

  • EU548082  Solanum tuberosum clone GKCM-TU02 granule-bound starch synthase (waxy) gene, partial cds     1461 bp    DNA   Submitted (01-MAR-2008)  AUTHORS: Bansal, A., Kumari, V., Sayal, R. and Das, N.

  • X70336  B. campestris (ACP-SF1) gene for acyl carrier protein  3955 bp  DNA   Submitted (08-FEB-1993)  AUTHORS: Das, N. and Ghosh, S.

  • X70337  B. campestris (ACP-SF2) gene for acyl carrier protein  2073 bp   DNA  Submitted (08-FEB-1993)  AUTHORS: Das, N. and Ghosh, S.

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