Debajyoti Dutta

Lecturer (Contractual)

Specialization

Biotechnology

Email

debajyoti.dutta@thapar.edu

Specialization

Biotechnology

Email

debajyoti.dutta@thapar.edu

• Department and Institution: Department of Biotechnology, Haldia Institute of Technology, West Bengal University of Technology, West Bengal, India.

Achievements

  1. Education
    Ph.D (Structural Biology) 2007 - 2013

    • Department and Institution: Department of Biotechnology, Indian Institute of Technology

      Kharagpur, West Bengal, India.

    • Supervisor: Prof. Amit Kumar Das

    • Thesis title: Crystal Structures of Mycobacterial and Staphylococcal Ketoacyl Reductases and Their

      Structure-Function Relationship.

    • Experience: Protein overexpression and purification in bacteria; macromolecular crystallography:

      crystallization of protein, crystallization of protein-inhibitor/substrate complexes, phasing molecular replacement and heavy atom; enzyme kinetics; CD and fluorescence spectroscopy; MALDI ToF, protein modeling and computational docking.

      B.Tech Hons. (Biotechnology) (DGPA 8.5/10) 2002 - 2006

    • Developed skills and protocols on styrene malic acid (SMA) copolymer based membrane protein

      purification and reconstitution into liposome.

    • Secured laboratory funding by designing projects and helped writing grants with supervisors.

    • Developed skills on protein computational software in Linux, OSX, WINDOWS and SGI

      environment.

    • Collaborated with inter and intra-lab mates to maximize the experience and research output.

    • Established, developed and maintained macromolecular diffraction facility and structure solution

      methods at Indian Institute of Technology Kharagpur in Prof. Amit Kumar Das Lab.

    • Achieved Graduate Aptitude Test in Engineering (GATE 2006) in Life Sciences (96 percentile).

    • Achieved West Bengal Joint Entrance Examination (WBJEE 2002) in Engineering (94 percentile).

  • Publications

    • Nakatani Y, Shimaki Y, Dutta D, Muench SP, Ireton K, Cook GM, Jeuken LJC. Unprecedented mode of action of phenothiazines as ionophores unravelled by an NDH-2 bioelectrochemical assay platform. J Am Chem Soc. 2020. Jan 22;142(3):1311-1320. doi: 10.1021/jacs.9b10254. PMID: 31880924.
      (IF 14.69, Citations NA)

      Dutta D, Esmaili E, Overduin M, Fliegel L. Expression and detergent free purification and reconstitution of the plant plasmamembrane Na+/H+ antiporter SOS1 overexpressed in Pichia pastoris. Biochim Biophys Acta Biomembranes. 2020 Mar 1;1862(3):183111. doi: 10.1016/j.bbamem.2019.183111. PMID: 31678368.

      (IF 3.7, Citations NA).

      Debajyoti Dutta, Ph.D

      Dutta D, Ullah A, Bibi S, Fliegel L. Functional Analysis of Conserved Transmembrane Charged Residues and a Yeast Specific Extracellular Loop of the Plasma Membrane Na+/H+ Antiporter of Schizosaccharomyces pombe. Sci Rep. 2019 Apr 17;9(1):6191. doi: 10.1038/s41598-019-42658-0. PMID: 30996236.

      (IF 4.12, Citations 2)

      Biswas R, Singh BK, Dutta D, Das PK, Maiti MK, Basak A, Das AK. Decrypting the oscillating nature of the 4'-phosphopantetheine arm in acyl carrier protein AcpM of Mycobacterium tuberculosis. FEBS Lett. 2019. Mar;593(6):622-633. doi: 10.1002/1873-3468.13339. PMID: 30847903.
      (IF 2.99, Citations 2)

      Wong KY, McKay R, PhD, Liu Y, Towle K, Elloumi Y, Li, X, Quan S, Dutta D, Sykes BD, Fliegel L. Diverse Residues of Intracellular Loop 5 of the Na+/H+ Exchanger Modulate Proton Sensing, Expression, Activity and Targeting. Biochim Biophys Acta Biomembranes. 2019. Jan;1861(1):191-200. doi: 10.1016/j.bbamem.2018.07.014. PMID: 30071192.

      (IF 3.43, Citations 3)

      Dutta D, Fliegel L. Molecular Modeling and Inhibitor Docking Analysis of the Na+/H+ Exchanger Isoform One. Biochem Cell Biol. 2019. Jun;97(3):333-343. doi: 10.1139/bcb-2018-0158. PMID: 30058365.
      (IF 1.8, Citations 3)

      Dutta D. Advance in Research on Mycobacterium tuberculosis FabG4 and Its Inhibitor. Front Microbiol. 2018 Jun 6;9:1184. doi: 10.3389/fmicb.2018.01184. PMID: 29946302.
      (IF 4.07, Citations NA)

      Biswas R, Singh B, Dutta D, Basak A, Das AK. Domain swapping between FabGs deciphers the structural determinant for in-solution oligomerization and substrate binding. Biophys Chem. 2018 Jun;237:9-21. doi: 10.1016/j.bpc.2018.03.003. PMID: 29625337.
      (IF 2.4, Citations 1)

      Dutta D, Fliegel L. Structure and function of yeast and fungal Na+ /H+ antiporters. IUBMB Life. 2018 Jan;70(1):23-31. doi: 10.1002/iub.1701. PMID: 29219228.
      (IF 3.1, Citations 8)

      Dutta D, Shin K, Rainey JK, Fliegel L. Transmembrane Segment XI of the Na+/H+ Antiporter of S. pombe is a Critical Part of the Ion Translocation Pore. Sci Rep. 2017 Oct 16;7(1):12793. doi: 10.1038/s41598-017-12701-z. PMID: 29038548.
      (IF 4.25, Citations 5)

      Lyons B, Ravulapalli R, Lanoue J, Lugo MR, Dutta D, Carlin S, Merrill AR. Scabin, a Novel DNA- acting ADP-ribosyltransferase from Streptomyces scabies. J Biol Chem. 2016 May 20;291(21):11198- 215. doi: 10.1074/jbc.M115.707653. PMID: 27002155.
      (IF 4.57, Citations 22)

      Ullah A, Dutta D, Fliegel L. Expression and characterization of the SOS1 Arabidopsis salt tolerance protein. Mol Cell Biochem. 2016 Apr;415(1-2):133-43. doi: 10.1007/s11010-016-2685-2. PMID: 26992907.
      (IF 2.39, Citations 12)

      Bhattacharyya S, Dutta A, Dutta D, Ghosh AK, Das AK. Structural elucidation of the NADP(H) phosphatase activity of staphylococcal dual-specific IMPase/NADP(H) phosphatase. Acta Crystallogr D Struct Biol. 2016 Feb;72(Pt 2):281-90. doi: 10.1107/S2059798316000620. PMID: 26894675.
      (IF 2.68, Citations 3)

      Debajyoti Dutta, Ph.D

      Lugo MR, Ravulapalli R, Dutta D, Rod Merrill A. Structural variability of C3larvin toxin. Intrinsic dynamics of the α/β fold of the C3-like group of mono-ADP-ribosyltransferase toxins. J Biomol Struct Dyn. 2016 Dec;34(12):2537-2560. doi: 10.1080/07391102.2015.1123189. PMID: 26610041.
      (IF 2.9, Citations 9)

      Biswas R, Dutta A, Dutta D, Hazra D, Banerjee DR, Basak A, Das AK. Crystal structure of dehydratase component HadAB complex of mycobacterial FAS-II pathway. Biochem Biophys Res Commun. 2015 Mar 6;458(2):369-74. doi: 10.1016/j.bbrc.2015.01.119. PubMed PMID: 25656575.
      (IF 2.29, Citations 11)

      Dutta A, Bhattacharyya S, Dutta D, Das AK. Structural elucidation of the binding site and mode of inhibition of Li(+) and Mg(2+) in inositol monophosphatase. FEBS J. 2014 Dec;281(23):5309-24. doi: 10.1111/febs.13070. PMID: 25263816.
      (IF 4.00, Citations 13)

      Banerjee DR, Dutta D, Saha B, Bhattacharyya S, Senapati K, Das AK, Basak A. Design, synthesis and characterization of novel inhibitors against mycobacterial β-ketoacyl CoA reductase FabG4. Org Biomol Chem. 2014 Jan 7;12(1):73-85. doi: 10.1039/c3ob41676c. PubMed PMID: 24129589.
      (IF 3.50, Citations 10)

      Biswas R, Dutta D, Das AK. Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of Rv0241c (HtdX) from Mycobacterium tuberculosis H37Rv. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Oct;69(Pt 10):1110-3. doi: 10.1107/S1744309113023452. PMID: 24100560.

      (IF 0.53, Citations 5)

      Dutta D, Bhattacharyya S, Roychowdhury A, Biswas R, Das AK. Crystal structure of hexanoyl-CoA bound to β-ketoacyl reductase FabG4 of Mycobacterium tuberculosis. Biochem J. 2013 Feb 15;450(1):127-39. doi: 10.1042/BJ20121107. PMID: 23163771.
      (IF 4.40, Citations 31)

      Mukherjee S, Roychowdhury A, Dutta D, Das AK. Crystal structures of triosephosphate isomerase from methicillin resistant Staphylococcus aureus MRSA252 provide structural insights into novel modes of ligand binding and unique conformations of catalytic loop. Biochimie. 2012 Dec;94(12):2532-44. doi:10.1016/j.biochi.2012.07.001. PMID: 22813930.

      (IF 2.96, Citations 7)

      Dutta D, Bhattacharyya S, Das AK. Crystallization and preliminary X-ray diffraction analysis of the high molecular weight ketoacyl reductase FabG4 complexed with NADH. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):786-9. doi: 10.1107/S1744309112020301. Epub 2012 Jun 27. Erratum in: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1417. PMID: 22750865.

      (IF 0.53, Citations 4)

      Dutta D, Bhattacharyya S, Das AK. Crystal structure and fluorescence studies reveal the role of helical dimeric interface of staphylococcal FabG1 in positive cooperativity for NADPH. Proteins. 2012 Apr;80(4):1250-7. doi: 10.1002/prot.24024. PMID: 22275129.
      (IF 2.60, Citations 22)

      Bhattacharyya S, Dutta D, Saha B, Ghosh AK, Das AK. Crystal structure of Staphylococcal dual specific inositol monophosphatase/NADP(H) phosphatase (SAS2203) delineates the molecular basis of substrate specificity. Biochimie. 2012 Mar;94(3):879-90. doi: 10.1016/j.biochi.2011.12.007. PMID: 22197784.
      (IF 2.96, Citations 12)

      Debajyoti Dutta, Ph.D

      Chandra K, Dutta D, Mitra A, Das AK, Basak A. Design, synthesis and inhibition activity of a novel cyclic enediyne amino acid conjugates against MPtpA. Bioorg Med Chem. 2011 May 15;19(10):3274-9. doi: 10.1016/j.bmc.2011.03.024. PMID: 21530278.
      (IF 2.79, Citations 8)

      Bhattacharyya S, Dutta D, Ghosh AK, Das AK. Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of an inositol monophosphatase family protein (SAS2203) from Staphylococcus aureus MSSA476. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt 4):471-4. doi: 10.1107/S1744309111003496. PMID: 21505244.

      (IF 0.53, Citations 3)

      Dutta D, Bhattacharyya S, Mukherjee S, Saha B, Das AK. Crystal structure of FabG4 from Mycobacterium tuberculosis reveals the importance of C-terminal residues in ketoreductase activity. J Struct Biol. 2011 Apr;174(1):147-55. doi: 10.1016/j.jsb.2010.11.012. PMID: 21081168.
      (IF 3.23, Citations 16)

      Chandra K, Dutta D, Das AK, Basak A. Design, synthesis and inhibition activity of novel cyclic peptides against protein tyrosine phosphatase A from Mycobacterium tuberculosis. Bioorg Med Chem. 2010 Dec 1;18(23):8365-73. doi: 10.1016/j.bmc.2010.09.052. PubMed PMID: 21050767.
      (IF 2.79, Citations 19)

      Mukherjee S, Dutta D, Saha B, Das AK. Crystal structure of glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylococcus aureus MRSA252 provides novel insights into substrate binding and catalytic mechanism. J Mol Biol. 2010 Sep 3;401(5):949-68. doi: 10.1016/j.jmb.2010.07.002. PMID: 20620151.

      (IF 4.33, Citations 36)

      Mukherjee S, Saha B, Dutta D, Das AK. Purification, crystallization and preliminary X-ray analysis of apo glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) from methicillin-resistant Staphylococcus aureus (MRSA252). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1;66(Pt 5):506-8. doi: 10.1107/S1744309110007980. PMID: 20445245.

      (IF 0.53, Citations 1)

      Bhattacharyya S, Dutta D, Ghosh AK, Das AK. Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of an atypical two-cysteine peroxiredoxin (SAOUHSC_01822) from Staphylococcus aureus NCTC 8325. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt 11):1113-5. doi: 10.1107/S1744309109037580. PMID: 19923729.

      (IF 0.53, Citations NA)

      Mukherjee S, Maity S, Roy S, Ghorai S, Chakrabarti M, Agarwal R, Dutta D, Ghosh AK, Das AK. Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate dehydrogenase from Antheraea mylitta. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Sep 1;65(Pt 9):937-40. doi: 10.1107/S174430910903214X. PMID: 19724138.

      (IF 0.53, Citations 1)

      Mukherjee S, Dutta D, Saha B, Das AK. Expression, purification, crystallization and preliminary X-ray diffraction studies of triosephosphate isomerase from methicillin-resistant Staphylococcus aureus (MRSA252). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Apr 1;65(Pt 4):398-401. doi:10.1107/S1744309109010112. PMID: 19342791.

      (IF 0.53, Citations 2)

      Debajyoti Dutta, Ph.D

      Saha B, Mukherjee S, Dutta D, Das AK. Expression, purification, crystallization and preliminary X-ray diffraction analysis of the transcriptional repressor SirR from Mycobacterium tuberculosis H37Rv. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Feb 1;65(Pt 2):154-8. doi: 10.1107/S1744309108043534. PMID: 19194009.

      (IF 0.53, Citations 2)

      Mukherjee S, Dutta D, Saha B, Das AK. Expression, purification, crystallization and preliminary X-ray diffraction studies of glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylococcus aureus (MRSA252). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Oct 1;64(Pt 10):929-32. doi: 10.1107/S1744309108027504. PMID: 18931438.

      (IF 0.53, Citations 4)

      Dutta D, De D, Chaudhuri S, Bhattacharya SK. Hydrogen production by Cyanobacteria. Microb Cell Fact. 2005 Dec 21;4:36. doi: 10.1186/1475-2859-4-36. PMID: 16371161.
      (IF NA, Citations 286)

      De D, Dutta D, Kundu M, Mahato S, Schiavone MT, Chaudhuri S, Giri A, Gupta V, Bhattacharya SK. Inactive enzymatic mutant proteins (phosphoglycerate mutase and enolase) as sugar binders for ribulose- 1,5-bisphosphate regeneration reactors. Microb Cell Fact. 2005 Feb 2;4(1):5. doi: 10.1186/1475-2859-4- 5. PMID: 15689239.

      (IF NA, Citations 1)

      Mahato S, De D, Dutta D, Kundu M, Bhattacharya S, Schiavone MT, Bhattacharya SK. Potential use of sugar binding proteins in reactors for regeneration of CO2 fixation acceptor D-Ribulose-1,5 bisphosphate. Microb Cell Fact. 2004 Jun 2;3(1):7. doi: 10.1186/1475-2859-3-7. PMID: 15175111.
      (IF NA, Citations 9)

      Poster Presentations in Conference

      Dutta D, Mazurenko I, Bridges HR, Fedor JG, Hirst J, Jeuken LJC, “Investigation of Proton Pumping by Bovine Complex I Using Solid Supported Membrane Electrophysiology” Xmas Bioenergetics 2019 program, Queen Mary University of London, UK, December 16, 2019.

      Dutta D, Esmaili M, Overduin M, Fliegel L, “Expression and Purification of the Plant Plasmamembrane Na+/H+ antiporter SOS1” 61st Annual meeting program Canadian Society for Molecular Biosciences, Banff, Alberta Canada, April 11-15, 2018.

      Dutta D, Ullah A, Fliegel L, “Structural and Functional Characterization of TM XI of spSod2” PDFA Research Day, University Of Alberta, Canada, October 2015.

      Dutta D, De D, Chowdhuri S, Bhattacharya S, “Photobioreactor Systems for Hydrogen Production by Cyanobacteria” in International conference on molecules to materials ICMM-2006 Sant Longowal Institute of Engineering and Technology, Longowal, Sangrur, Punjab, India, March 2006.