Dr. Debajyoti Dutta

Assistant Professor


Protein Engineering, Membrane Proteins, Downstream Processing




Protein Engineering, Membrane Proteins, Downstream Processing





Research Interests :

Membrane Proteins; Recombinant Membrane Protein Expression and Characterization; Protein Engineering


Education :

Ph.D., Protein Crystallography & Structural Biology 2013

Indian Institute of Technology Kharagpur,


B.Tech, Biotechnology 2006

West Bengal University of Technology


Research Experience :

Research Fellow, University of Leeds, UK                      2019 - 2020

Postdoctoral Fellow, University of Alberta, Canada         2014 - 2019

Postdoctoral Fellow, University of Guelph, Canada         2013 - 2014


Other Research Experiences :

  • Visiting Postdoctoral Fellow at Mitochondrial Biology Unit, University of Cambridge, UK.
  • Visiting Postdoctoral Research Exchange Fellow at Plant Physiology division, Technische Unie Kaiserslautern, Germany.
  • Pre-Ph.D. Research Fellow at Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, USA.

Research Grants :

  • Development of crop salinity stress markers using plant protein related to salt tolerance. (PI), 2022 - 2023, TIET seed grant

  • Identification of immunogenic peptide for diagnosis of otrthohantavirus infection. (CoPI) 2022 - 25, DST Indo-Russia

  • Development of native-lipid nanodisc mediated protocol for the identification of crop salinity stress associated plasma membrane proteins and lipid interactions. (PI) 2022 - 2024, SERB SRG


Awards and Fellowship :

  • Postdoctoral fellow MemStar Award for best publication, Membrane Protein Disease Research Group, University of Alberta, Canada.
  • Received NSERC International Research Training Group in Membrane Biology Fellowship at University of Alberta, Canada.
  • Awarded travel grant to attain workshops on computational crystallography and synchrotron diffraction data collection in EMBL Hamburg, Germany (DESSY).

Patent filed :

  • Banerjee D, Dutta D, Saha B, Das AK, Basak A. “1,4 triazole based polyphenol hybrids”. Dec 2014 (Patent application no. 633/KOL/2013). (Patent filed in India) 


Selected Publications :

For detailed publication can be found in PUBMED 




Li X, Kim J, Yang J, Dutta D, Fliegel L. Characterization of modeled inhibitory binding sites on isoform one of the Na +/H + exchanger. Biochim Biophys Acta Biomembr. 2021. 1863(9):183648. doi: 10.1016/j.bbamem.2021.183648.


Blair S, Li X, Dutta D, Chamot D, Fliegel L, Goss G. Rainbow Trout ( Oncorhynchus mykiss) Na +/H + Exchangers tNhe3a and tNhe3b Display Unique Inhibitory Profiles Dissimilar from Mammalian NHE Isoforms. Int J Mol Sci. 2021. 22(4):2205. doi: 10.3390/ijms22042205.


Li X, Dutta D, Jung M, Zimmermann R, Fliegel L. Amino Acids 563-566 of the Na +/H + Exchanger Isoform 1 C-Terminal Cytosolic Tail Prevent Protein Degradation and Stabilize Protein Expression and Activity. Int J Mol Sci. 2020. 21(5):1737. doi: 10.3390/ijms21051737.


Other selected publications


Nakatani Y, Shimaki Y, Dutta D, Muench SP, Ireton K, Cook GM, Jeuken LJC. Unprecedented mode of action of phenothiazines as ionophores unravelled by an NDH-2 bioelectrochemical assay platform. J Am Chem Soc. 2020. Jan 22;142(3):1311-1320. doi: 10.1021/jacs.9b10254. PMID: 31880924.


Dutta D, Esmaili E, Overduin M, Fliegel L. Expression and detergent free purification and reconstitution of the plant plasmamembrane Na+/H+ antiporter SOS1 overexpressed in Pichia pastoris. Biochim Biophys Acta Biomembranes. 2020 Mar 1;1862(3):183111. doi: 10.1016/j.bbamem.2019.183111. PMID: 31678368.


Dutta D, Ullah A, Bibi S, Fliegel L. Functional Analysis of Conserved Transmembrane Charged Residues and a Yeast Specific Extracellular Loop of the Plasma Membrane Na+/H+ Antiporter of Schizosaccharomyces pombe. Sci Rep. 2019 Apr 17;9(1):6191. doi: 10.1038/s41598-019-42658-0. PMID: 30996236.


Dutta D, Fliegel L. Molecular Modeling and Inhibitor Docking Analysis of the Na+/H+ Exchanger Isoform One. Biochem Cell Biol. 2019. Jun;97(3):333-343. doi: 10.1139/bcb-2018-0158. PMID: 30058365.


Dutta D. Advance in Research on Mycobacterium tuberculosis FabG4 and Its Inhibitor. Front Microbiol. 2018 Jun 6;9:1184. doi: 10.3389/fmicb.2018.01184. PMID: 29946302.


Dutta D, Fliegel L. Structure and function of yeast and fungal Na+ /H+ antiporters. IUBMB Life. 2018 Jan;70(1):23-31. doi: 10.1002/iub.1701. PMID: 29219228.


Dutta D, Shin K, Rainey JK, Fliegel L. Transmembrane Segment XI of the Na+/H+ Antiporter of S. pombe is a Critical Part of the Ion Translocation Pore. Sci Rep. 2017 Oct 16;7(1):12793. doi: 10.1038/s41598-017-12701-z. PMID: 29038548.


Ullah A, Dutta D, Fliegel L. Expression and characterization of the SOS1 Arabidopsis salt tolerance protein. Mol Cell Biochem. 2016 Apr;415(1-2):133-43. doi: 10.1007/s11010-016-2685-2. PMID: 26992907.


Dutta D, Bhattacharyya S, Roychowdhury A, Biswas R, Das AK. Crystal structure  of hexanoyl-CoA bound to β-ketoacyl reductase FabG4 of Mycobacterium tuberculosis. Biochem J. 2013 Feb 15;450(1):127-39. doi: 10.1042/BJ20121107. PMID: 23163771.


Dutta D, Bhattacharyya S, Das AK. Crystallization and preliminary X-ray diffraction analysis of the high molecular weight ketoacyl reductase FabG4 complexed with NADH. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):786-9. doi: 10.1107/S1744309112020301. Epub 2012 Jun 27. Erratum in: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1417. PMID: 22750865.


Dutta D, Bhattacharyya S, Das AK. Crystal structure and fluorescence studies reveal the role of helical dimeric interface of staphylococcal FabG1 in positive cooperativity for NADPH. Proteins. 2012 Apr;80(4):1250-7. doi: 10.1002/prot.24024. PMID: 22275129.


Dutta D, Bhattacharyya S, Mukherjee S, Saha B, Das AK. Crystal structure of FabG4 from Mycobacterium tuberculosis reveals the importance of C-terminal residues in ketoreductase activity. J Struct Biol. 2011 Apr;174(1):147-55. doi: 10.1016/j.jsb.2010.11.012. PMID: 21081168.


Mukherjee S, Dutta D, Saha B, Das AK. Crystal structure of glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylococcus aureus MRSA252 provides novel insights into substrate binding and catalytic mechanism. J Mol Biol. 2010 Sep 3;401(5):949-68. doi: 10.1016/j.jmb.2010.07.002. PMID: 20620151.


Dutta D, De D, Chaudhuri S, Bhattacharya SK. Hydrogen production by Cyanobacteria. Microb Cell Fact. 2005 Dec 21;4:36. doi: 10.1186/1475-2859-4-36. PMID: 16371161.

Download Brochure