Specialization

Protein Engineering, Membrane Proteins, Downstream Processing

Email

debajyoti.dutta@thapar.edu

Personal Webpage

 

Research Interest :

 

Abiotic stress, including salinity, is a severe challenge in agricultural production. Our lab focuses on membrane protein's role in plant abiotic stress tolerance.

1. Membrane Proteins:

We use structural and functional information of plant membrane proteins to understand and develop plant salinity tolerance.

2. Bioproduction and Downstream Processing

We also have interests in recombinant membrane protein/polypeptide production, purification, and characterization.

3. Protein Engineering

Structure-based protein engineering is our third research direction, where we attempt to design, recombinant production, and characterization of engineered proteins with an evolved function.

 

Education :

Ph.D., Indian Institute of Technology Kharagpur, 2013

Protein Crystallography & Structural Biology

 

B.Tech., West Bengal University of Technology, 2006

Biotechnology

 

 

Research Experience :

Research Fellow, University of Leeds, UK                        2019 - 2020

Postdoctoral Fellow, University of Alberta, Canada         2014 - 2019

Postdoctoral Fellow, University of Guelph, Canada         2013 - 2014

 

Other Research Experiences :

• Visiting Postdoctoral Fellow at Mitochondrial Biology Unit, University of Cambridge, UK.

• Visiting Postdoctoral Research Exchange Fellow at Plant Physiology division, Technische Unie Kaiserslautern, Germany.

• Pre-Ph.D. Research Fellow at Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, USA.

 

Courses Developed and Taught :

 

Structural Biology (UBT512)

Protein Engineering (UBT616)

Protein Engineering & Enzyme Technology (UBT623)

Structural Biology & Protein Engineering (PBY109)

Protein Engineering (PBT315)

 

Research Grants :

• Thapar Institute of Engineering & Technology Seed Grant,  (PI), 2021 - 2023

• Department of Science & Technology Government. of India, Indo-Russia, (CoPI) 2023 - 2026

• Science and Engineering Research Board, StartUp Research Grant, (PI) 2022 - 2024

 

Awards and Fellowship :

• Postdoctoral fellow MemStar Award for best publication, Membrane Protein Disease Research Group, University of Alberta, Canada.

• Received NSERC International Research Training Group in Membrane Biology Fellowship at University of Alberta, Canada.

• Awarded travel grant to attain workshops on computational crystallography and synchrotron diffraction data collection in EMBL Hamburg, Germany (DESSY).

Patent filed :

• Banerjee D, Dutta D, Saha B, Das AK, Basak A. “1,4 triazole based polyphenol hybrids”. Dec 2014 (Patent application no. 633/KOL/2013). (Patent filed in India) 

 

Selected Publications :

For detailed publication can be found in PUBMED 

 

From TIET

Journal publications:

 

Das PK, Bhatnagar T, Banik S, Majumdar S, Dutta D, Structural and Molecular Dynamics Simulation studies of CBL-interacting protein kinase CIPK and its complexes related to plant salinity stress. J Mol Model. 2024. 30(8):248. https://doi.org/10.1007/s00894-024-06037-5

 

Book Chapter:

Dutta D, Das PK. Application of CRISPR Technology in Plant Salt Tolerance and Membrane Proteins. (2024) Chapter in Application of CRISPR Technology in Plant Salt Tolerance and Membrane Proteins, Edited by Anshu Alok, Jitesh Kumar, Mahipal Shekhawat, pp 224 - 247, CRC Press.

 

Journal publications:

 

Dutta D, Interplay between membrane proteins and membrane protein-lipid pertaining to plant salinity stress. Cell Biochem & Funct. 2023. 41(4)399-412. https://doi.org/10.1002/cbf.3798

 

Banik S, Dutta D, Membrane proteins in plant salinity stress perception, sensing and response. J Membr Biol. 2023. 256(2) 109-124. https://doi.org/10.1007/s00232-023-00279-9

 

Li X, Kim J, Yang J, Dutta D, Fliegel L. Characterization of modeled inhibitory binding sites on isoform one of the Na +/H + exchanger. Biochim Biophys Acta Biomembr. 2021. 1863(9):183648. doi: 10.1016/j.bbamem.2021.183648.

 

Blair S, Li X, Dutta D, Chamot D, Fliegel L, Goss G. Rainbow Trout ( Oncorhynchus mykiss) Na +/H + Exchangers tNhe3a and tNhe3b Display Unique Inhibitory Profiles Dissimilar from Mammalian NHE Isoforms. Int J Mol Sci. 2021. 22(4):2205. doi: 10.3390/ijms22042205.

 

Li X, Dutta D, Jung M, Zimmermann R, Fliegel L. Amino Acids 563-566 of the Na +/H + Exchanger Isoform 1 C-Terminal Cytosolic Tail Prevent Protein Degradation and Stabilize Protein Expression and Activity. Int J Mol Sci. 2020. 21(5):1737. doi: 10.3390/ijms21051737.

 

Other selected publications

 

Nakatani Y, Shimaki Y, Dutta D, Muench SP, Ireton K, Cook GM, Jeuken LJC. Unprecedented mode of action of phenothiazines as ionophores unravelled by an NDH-2 bioelectrochemical assay platform. J Am Chem Soc. 2020. Jan 22;142(3):1311-1320. doi: 10.1021/jacs.9b10254. PMID: 31880924.

 

Dutta D, Esmaili E, Overduin M, Fliegel L. Expression and detergent free purification and reconstitution of the plant plasmamembrane Na⁺/H⁺ antiporter SOS1 overexpressed in Pichia pastoris. Biochim Biophys Acta Biomembranes. 2020 Mar 1;1862(3):183111. doi: 10.1016/j.bbamem.2019.183111. PMID: 31678368.

 

Dutta D, Ullah A, Bibi S, Fliegel L. Functional Analysis of Conserved Transmembrane Charged Residues and a Yeast Specific Extracellular Loop of the Plasma Membrane Na+/H+ Antiporter of Schizosaccharomyces pombe. Sci Rep. 2019 Apr 17;9(1):6191. doi: 10.1038/s41598-019-42658-0. PMID: 30996236.

 

Dutta D, Fliegel L. Molecular Modeling and Inhibitor Docking Analysis of the Na+/H+ Exchanger Isoform One. Biochem Cell Biol. 2019. Jun;97(3):333-343. doi: 10.1139/bcb-2018-0158. PMID: 30058365.

 

Dutta D. Advance in Research on Mycobacterium tuberculosis FabG4 and Its Inhibitor. Front Microbiol. 2018 Jun 6;9:1184. doi: 10.3389/fmicb.2018.01184. PMID: 29946302.

 

Dutta D, Fliegel L. Structure and function of yeast and fungal Na+ /H+ antiporters. IUBMB Life. 2018 Jan;70(1):23-31. doi: 10.1002/iub.1701. PMID: 29219228.

 

Dutta D, Shin K, Rainey JK, Fliegel L. Transmembrane Segment XI of the Na+/H+ Antiporter of S. pombe is a Critical Part of the Ion Translocation Pore. Sci Rep. 2017 Oct 16;7(1):12793. doi: 10.1038/s41598-017-12701-z. PMID: 29038548.

 

Ullah A, Dutta D, Fliegel L. Expression and characterization of the SOS1 Arabidopsis salt tolerance protein. Mol Cell Biochem. 2016 Apr;415(1-2):133-43. doi: 10.1007/s11010-016-2685-2. PMID: 26992907.

 

Dutta D, Bhattacharyya S, Roychowdhury A, Biswas R, Das AK. Crystal structure  of hexanoyl-CoA bound to β-ketoacyl reductase FabG4 of Mycobacterium tuberculosis. Biochem J. 2013 Feb 15;450(1):127-39. doi: 10.1042/BJ20121107. PMID: 23163771.

 

Dutta D, Bhattacharyya S, Das AK. Crystallization and preliminary X-ray diffraction analysis of the high molecular weight ketoacyl reductase FabG4 complexed with NADH. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):786-9. doi: 10.1107/S1744309112020301. Epub 2012 Jun 27. Erratum in: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1417. PMID: 22750865.

 

Dutta D, Bhattacharyya S, Das AK. Crystal structure and fluorescence studies reveal the role of helical dimeric interface of staphylococcal FabG1 in positive cooperativity for NADPH. Proteins. 2012 Apr;80(4):1250-7. doi: 10.1002/prot.24024. PMID: 22275129.

 

Dutta D, Bhattacharyya S, Mukherjee S, Saha B, Das AK. Crystal structure of FabG4 from Mycobacterium tuberculosis reveals the importance of C-terminal residues in ketoreductase activity. J Struct Biol. 2011 Apr;174(1):147-55. doi: 10.1016/j.jsb.2010.11.012. PMID: 21081168.

 

Mukherjee S, Dutta D, Saha B, Das AK. Crystal structure of glyceraldehyde-3-phosphate dehydrogenase 1 from methicillin-resistant Staphylococcus aureus MRSA252 provides novel insights into substrate binding and catalytic mechanism. J Mol Biol. 2010 Sep 3;401(5):949-68. doi: 10.1016/j.jmb.2010.07.002. PMID: 20620151.

 

Dutta D, De D, Chaudhuri S, Bhattacharya SK. Hydrogen production by Cyanobacteria. Microb Cell Fact. 2005 Dec 21;4:36. doi: 10.1186/1475-2859-4-36. PMID: 16371161.